Signs and symptoms shrimps lethargic with surface and gill fouling. Decontamination of monodon baculovirus in marine shrimp. At the individual level, quantitative histopathology was utilized to differentiate monodon baculovirus mbv in the digestive tissues the midgut or mg and. The former species is indigenous in most asian countries while the latter is indigenous in the americas and was introduced to asian countries in the late. Prevalence of white spot syndrome virus wssv and monodon baculovirus mbv infection in penaeus monodon postlarvae in vietnam. Detection of monodon baculovirus and whitespot syndrome. It is a rodshaped, enveloped, doublestranded dna virus, and considered till recently, as the type a baculovirus. Aquaculture elsev1er aquaculture l 995 1295 a record and prevalence omonodon baculovirus from postlarval penaeus monodon in madras, india p.
Identification of white spot syndrome associated baculovirus wsbv target organs in the shrimp penaeus monodon by in situ hybridization pohshing changl, chufang l o yuchi, wang2,guanghsiung k o u v department of aquaculture, national kaohsiung institute of marine technology, kaohsiung, taiwan, roc 2 e p a r t m e nof t zoology. Walker cooperative research centre for aquaculture, csiro tropical agriculture, long pocket laboratories, pmb3, indooroopilly 4068, australia. In tropical asia, the two main species of penaeid shrimps that are widely cultured are the black tiger shrimp penaeus monodon and the pacific white shrimp penaeus litopenaeus vannamei. Pdf prevalence of white spot syndrome virus and monodon. The nomenclature and classification status of pmnv has been changed several times due to morphological. Isolation and molecular identification of planctomycete. The cytopathology, virogenesis and replication of monodon baculovirus mbv in penaeus monodon from australia are described. The broodstock were collected along the coast of tamil. Two years later, it was designated monodon baculovirus mbv 2, and this name is still commonly used today. Monodon baculovirus mbv is a major virus that infects the. White spot syndrome virus wssv infection was induced in tiger shrimp, penaeus monodon, under laboratory conditions, and histopathological changes in subcuticular epithelial cells of the eye stalk and pleopod were studied sequentially at different time postchallenge.
Mbv monodon baculovirus shrimp care aquaculture products. The objective of our study was to explain the histopathological changes of monodon baculovirus mbv in hepatopancreas and midgut tissues of the cultured penaeus vannamei. The results showed that mbv may significantly retard larval growth and cause mortalities during the larval stage. Guide to baculovirus expression vector systems bevs and. Besides mbv, two strains of snpv are reportedplebejus. Penaeus monodon is the secondmost widely cultured prawn species in the world, after only whiteleg shrimp, litopenaeus vannamei.
Recently, a number of advances have been made in the characteriza tion of the replication of baculovirus dna, including the identification. Nudiviridae is a virus family newly approved by the international committee on taxonomy of viruses ictv containing nonoccluded baculoviruses that previously had been placed in the family baculoviridae because of their large, doublestranded dna genome, replication in the nucleus, and hypertrophy of infected cells. Penaeus monodon by in situ hybridization national taiwan. This is the third edition of a book that was initiated with the annotation of the function of all the genes in the most commonly studied baculovirus, acmnpv.
At the individual level, quantitative histopathology was utilized to differentiate monodon baculovirus mbv in the digestive tissues the midgut or mg and hepatopancreas or hp of australian penaeus monodon and penaeus merguiensis. Baculovirus dna replication 857 p10, which encodes a small poorly conserved protein that may be in volved in occlusion body formation or cell lysis van oers et al. Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain bacteria were isolated from postlarvae of the giant tiger prawn, penaeus monodon. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by nested polymerase chain reaction pcr. Apr 14, 2003 the simultaneous presence of monodon baculovirus mbv and white spot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by nested polymerase chain reaction pcr. Pdf baculovirus infection in brackishwater pond cultured. Traditional baculovirus systems require purification and amplification of an initial lowtiter viral supernatant. Pdf prevalence of white spot syndrome virus wssv and.
Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. The interaction of prawns, viruses and the environment in disease development at individual and population levels was studied using histological and statistical analyses based on quantitative models and simple quantitative methods. The present invention relates to the establishment of an immortal shrimp cell line pmo derived from penaeus monodon. Secondary bacterial and epicommensal infections were also noted in many cases. In the meantime, mortality for each species of shrimps was recorded. Viral infection was associated with cumulative mortalities of greater than 90% 2 weeks after the appearance of the. Prevalence of mbv infection was found to be as high as 42% in a few localities.
Prevalence of white spot syndrome virus and monodon baculovirus in penaeus monodon broodstock and postlarvae from hatcheries. Penaeus monodon type baculovirus mbv was diagnosed for the first time in p. Pdf decontamination of monodon baculovirus in marine shrimp. Us6143547a cell line derived from penaeus monodon and a. Monodon baculovirus mbv infects the freshwater prawn macrobrachium rosenbergii cultivated in thailand. More than 600 recombinant genes have been expressed in baculoviruses to date. A new baculovirus of cultured shrimps springerlink. A modified dna extraction procedure was also developed to circumvent problems associated with copurification of pcr.
Jul 25, 2014 penaeus monodon nudivirus pmnv is the causative agent of spherical baculovirosis in shrimp penaeus monodon. White spot baculovirus syndrome in the indian shrimp. Piyapong chotipuntu1, suwit wuthisuthimethavee1,2, sataporn direkbusrakom1,2. This book is based on a project to completely annotate what is known about the function of the 150 genes encoded by the genome of the most commonly investigated baculovirus, acmnpv. Prevalence of white spot syndrome virus and monodon baculovirus in penaeus monodon broodstock and postlarvae from hatcheries in southeast coast of india. Baculovirus expression system, bacpak, is a complete system for expressing fullyfunctional recombinant proteins at extremely high levels in insect host cells. Mbv is one of the widely reported and well described viral pathogens of shrimp. Prevalence and histopathology of monodon baculovirus mbv. Mbv monodon baculovirus the occlusion bodies ob formed as monodon baculovirus mbv replicate are easily visible using a microscope. Ultrastructural and pathogenesis of monodon baculovirus in.
Routine histological techniques using paraffin embedded tissues, as well as frozen tissues, were used to document. Penaeus monodon baculovirus variously known as penaeus monodon singly. Aug 25, 2012 among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Wsbv in captured brooders of penaeus monodon with a special emphasis on reproductive organ. Penaeus monodon baculovirus mbv 191 temperatures range of 2328z. Occurrence and pathology of penaeus monodon baculovirus. A 2year survey 199395 on diseases of cultured shrimp penoeus monodon and fi indicus in shrimp farms of tamil nadu and andhra pradesh, south india, revealed a high prevalence of monodon baculovirus mbv infection. Bactobac baculovirus expression system thermo fisher. The present study attempts to investigate the effect of penaeus monodon. Pdf detection and tissue tropism of white spot syndrome. This disease causes significant mortalities at the larval stage and early postlarval pl stage and may suppress growth and reduce survival and production in aquaculture.
Monodon baculovirus mbv is a member of nucleopolyhedrosis virus npv group that infect penaeid shrimps. Major viral diseases of the black tiger prawn penaeus. By means of ultrathin section, negative staining and sucrose gradient ultracentrifugation, a new baculovims has been discovered and purified in lymphoid organs and such tissues as muscles of the shrimps which have been spontaneously attacked by diseases and artificially infected. Baculovirus definition of baculovirus by medical dictionary. Mbv had been implicated as the causative agent of the first. Detection of monodon baculovirus and whitespot syndrome virus. Baculovirus dna 96299 esf 921 delta series insect cell culture medium, custom amino acid deficient 91100 bestbac 1. Samples from penaeus monodon hatcheries 23 day old larvae or zoea 1 to 34 day old post larvae or pl 34 and ponds 15 to 159 days old in the pond were histologically examined for the presence of eosinophilic occlusion bodies in hypertrophied nuclei of the hepatopancreas which is indicative of p. Mbv was first identified in 1977 from taiwanese shrimps 47, 56.
Mbv virions are rodshaped, nonoccluded or occluded and enveloped within a paracrystalline occlusion body. A selective medium designed in the light of planctomycete antibiotic resistance characteristics was used for this isolation. Quantitative histopathology and epidemiology of prawn. Evidence for the presence of white spot syndrome virus. There are five different viruses which are currently being studied for their impact on commercial farming of the black tiger prawn penaeus monodon in thailand. Since then it has been identified and reported from a variety of penaeid shrimp species from different parts of the world 3, 44, 50, 54, 58, 69, 70. Pdf monodon baculovirus mbv infects the freshwater. The successful farming of tiger shrimp penaeus monodon in india is mainly due to the existence of some 300 hatcheries whose capacity to produce 12 000 million postlarvae pl annually has provided an assured supply of seed. Detection of monodon baculovirus and white spot syndrome virus in apparently healthy penaeus monodon postlarvae from india by polymerase chain reaction. Baculodirect baculovirus expression system makes baculovirus expression more convenient, requiring less hands on time than traditional systems.
In contrast, the bactobac baculovirus expression system is faster and easier because the technology relies on generation of recombinant baculovirus by sitespecific transposition in e. Baculovirus expression systems typically require bacterial transformation and isolation of a large bacmid or cotransfection of a transfer vector and linear baculovirus dna into insect cells. Planctomycetes were isolated from both healthy and monodon baculovirus. Prevalence of monodon baculovirus mbv in wild shrimp. Pdf decontamination of monodon baculovirus in marine. The broodstock were collected along the coast of tamil nadu and andhra pradesh during summer. Tetrahedral baculovirosis baculovirus penaei n450 crustacean. Download 597 kb abstract foreword acknowledgements abbreviations and acronyms. Detection of monodon baculovirus and white spot syndrome. Baculodirect baculovirus expression system thermo fisher. Decontamination of monodon baculovirus in marine shrimp eggs using upwelling flowthrough system. There appear to be more ob when pl are stressed, so the presence of ob can be taken as an indication of stress in the pl. Since 1985, when the first protein il2 was produced in large scale from a recombinant baculovirus, use of bevs has increased.
The genome and occlusion bodies of marine penaeus monodon. We use cookies to improve your browsing experience and provide meaningful content. As an occluded virus, this species would not be a nudivirus. Overview of the baculovirus expression system chambers. There is at least 1 species of baculovirus that does infect shrimp. Ultrastructural and pathogenesis of monodon baculovirus in spf transmission electron microscopy tem study on prepared samples of shrimps exposing to the virus was continued for transmission electron microscopy following afsharnasab 2007.
As variations in the strains of mbv would greatly affect the detection efficiency of pcr assays, the objective of this study was to. Jayakumar1 unit of cell biology and parasitology, department of zoology, life science building, university of madras, madras 600 025, india school of biology and biochemistry, medical biology centre, the. However, only 15% of samples were positive by nonnested reaction. Colourimetric pcrbased detection of monodon baculovirus in whole penaeus monodon postlarvae. Prevalence of monodon baculovirus mbv was determined using polymerase chain reaction pcr on deoxyribonucleic acid dna extracts from the hepatopancreas of the wild black tiger shrimp, penaeus monodon collected from 7 sampling sites in the philippines. Some of these viruses cause disease in other penaeid shrimp species and even other crustacean species. Environmental assessment report import of specific. Aug 25, 2012 among the viruses infecting penaeid shrimp, monodontype baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. White spot baculovirus syndrome in the indian shrimp penaeus monodon and p. Download fulltext pdf download fulltext pdf download fulltext pdf. Penaeus monodon baculovirus variously known as penaeus monodon.
My second suggestion of 21 december 20 may be disregarded. Research article open access the genome and occlusion. A survey on the presence of the viruses of two economically significant diseases, white spot syndrome virus wssv and monodon baculovirus mbv in wild. There are several advantages of using baculovirus expression system over e. Development of antiviral gene therapy for monodon baculovirus using dsrna loaded chitosandextran sulfate nanocapsule delivery system in penaeus monodon postlarvae.
Download fulltext pdf monodon baculovirus of shrimp article pdf available in indian journal of virology 232. Spherical baculovirosis penaeus monodon type baculovirus p. From each tank 20 postlarvae were randomly collected for the observation of mbv infection at each consecutive periods as indicated in fig. The simultaneous presence of monodon baculovirus mbv and white spot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by nested polymerase chain reaction pcr. Some occur not only in cultivated shrimp in other asian countries, but also in those from australia and the western hemisphere. Colourimetric pcrbased detection of monodon baculovirus. The second and third editions involved the update of this information and its integration into chapters covering the major processes central to the replication and pathology of baculoviruses. Quantitative histopathology and epidemiology of prawn viral. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Decontamination of monodon baculovirus in marine shrimp eggs. Penaeus monodon, nonoccluded baculovirus nob ii wongteerasupaya et al. For customers who prefer a eukaryotic expression system, are exploring lowercost alternatives to mammalian expression system but. Monodon baculovirus mbv exists in populations of the indian tiger prawn, penaeus monodon, obtained from a commercial hatchery in madras, india.
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